The antiserum was produced against synthesized phospho-peptide derived from human NPM around the phosphorylation site of Thr234.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
NPM (Phospho-Thr234) Antibody detects endogenous levels of NPM1 only when phosphorylated at Thr234.