The antiserum was produced against synthesized phospho-peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
SAPK/JNK (Phospho-Tyr185) Antibody detects endogenous levels of MAPK8/9/10 only when phosphorylated at Tyr185.