The antiserum was produced against synthesized phospho-peptide derived from human Src around the phosphorylation site of Tyr216.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
Src (Phospho-Tyr216) Antibody detects endogenous levels of SRC only when phosphorylated at Tyr216.