The antiserum was produced against synthesized phospho-peptide derived from human ACK around the phosphorylation site of Tyr518.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
ACK (Phospho-Tyr518) antibody detects endogenous levels of ACK only when phosphorylated at Tyr518.