The antiserum was produced against synthesized phospho-peptide derived from human c-Fos around the phosphorylation site of Thr325.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
c-Fos (Phospho-Thr325) antibody detects endogenous levels of c-Fos only when phosphorylated at Thr325.