The antiserum was produced against synthesized phospho-peptide derived from human Cortactin around the phosphorylation site of Tyr446.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
Cortactin (Phospho-Tyr446) antibody detects endogenous levels of Cortactin only when phosphorylated at Tyr446.