The antiserum was produced against synthesized phospho-peptide derived from human Hrs around the phosphorylation site of Tyr216.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
Hrs (Phospho-Tyr216) antibody detects endogenous levels of Hrs only when phosphorylated at Tyr216.