The antiserum was produced against synthesized phospho-peptide derived from human B-Raf around the phosphorylation site of Thr753.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
B-Raf (Phospho-Thr753) Antibody detects endogenous levels of BRAF1 only when phosphorylated at Thr753.