The antiserum was produced against synthesized phospho-peptide derived from human eNOS around the phosphorylation site of Thr494.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
eNOS (Phospho-Thr494) Antibody detects endogenous levels of NOS3 only when phosphorylated at Thr494.