The antiserum was produced against synthesized phospho-peptide derived from human Kir6.2 around the phosphorylation site of Thr224.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
Kir6.2 (Phospho-Thr224) Antibody detects endogenous levels of KCNJ11 only when phosphorylated at Thr224.