The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Thr254.
Immunogen Range
221-270
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
NF-kappaB p65 (Phospho-Thr254) Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at Thr254.