The antiserum was produced against synthesized phospho-peptide derived from human Flt-3 around the phosphorylation site of Tyr591.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
Flt-3 (Phospho-Tyr591) antibody detects endogenous levels of Flt-3 only when phosphorylated at Tyr591.