The antiserum was produced against synthesized phospho-peptide derived from human PP1alpha around the phosphorylation site of Thr320.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
PP1alpha (Phospho-Thr320) antibody detects endogenous levels of PP1alpha only when phosphorylated at Thr320.