The antiserum was produced against synthesized phospho-peptide derived from human MAP2K1 around the phosphorylation site of Thr386.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
MAP2K1 (Phospho-Thr386) Antibody detects endogenous levels of MAP2K1 only when phosphorylated at Thr386.