The antiserum was produced against synthesized phospho-peptide derived from human p27 Kip1 around the phosphorylation site of Thr187.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
p27 Kip1 (Phospho-Thr187) Antibody detects endogenous levels of CDKN1B only when phosphorylated at Thr187.