The antiserum was produced against synthesized phospho-peptide derived from human VE-Cadherin around the phosphorylation site of Tyr731.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
VE-Cadherin (Phospho-Tyr731) Antibody detects endogenous levels of CDH5 only when phosphorylated at Tyr731.